The Axion Lux3 FL is a small fluorescence live-cell imaging microscope equipped with one brightfield and two fluorescent channels (green and red). The device enables researchers to unravel cellular processes in real-time, providing lots of valuable data about the experiments and answers if, when, how, and to what extend the cellular processes occur. Axion’s first fluorescent cell imaging device allows users to track dynamic cellular processes with high specificity by taking high-quality images to create real-time time-lapse movies. Simultaneously, the cells are kept in a controlled environment inside a standard cell culture incubator.

Let Lux3 FL capture fluorescent and brightfield images for you

Currently, fluorescent labeling is mostly used as an end-point measurement. However, time-lapse imaging of live cells can give much more information about biological processes. By using automated imaging at regular time intervals, the temporal resolution of the fluorescent data is increased, leading to even more relevant data about the cellular processes. In this way, with fluorescence microscopy, researchers can not only determine if a certain process has occurred, but also when it occurred and at what speed.

Being able to monitor cell cultures over time provides a great insight into their physiology and function. Live-cell imaging microscopes open up novel and exciting avenues to study cellular health, viability, colony formation, migration, and cellular responses to external stimuli.

To help life science researchers improve their understanding of cellular processes, AXION BioSystems has developed an automated brightfield microscope that can visualize an entire surface of a cell culture vessel and operate from inside a standard CO2-incubator, biological safety cabinet, or on a benchtop. Not limited to a specific type or quantity of culture vessels, the AXION Omni captures cellular behavior by creating high-quality time-lapse videos for days or even weeks at a time.

Product outline:

• Stage Top Incubator for easy live cell imaging on every inverted microscope: Universal Fit with CO2 and O2 control
• Physiologic conditions directly on the microscope
• Precise control of temperature, humidity, CO2, and O2
• Compatible with all inverted microscopes that have a multiwell plate holder

A µ-Plate with 96 wells for high-throughput 3D cell culture and tube formation assays

A chambered coverslip with 8 wells for cell culture, immunofluorescence, and high-end microscopy

Investigate chemotaxis of fast or slow migrating adherent cells and non-adherent cells in 2D or 3D gel matrices

Product Outline:

• A µ-Slide with a porous glass membrane for transmigration and transport studies under both static and flow conditions
• Brilliant optical quality through the thin, porous glass membrane
• Full access to the apical and basal sides of adherent cells in numerous applications
• Different pore sizes available for various cell types
• Suitable for the establishment of lung models with air-liquid interface (ALI)

Applications

• Trans-endothelial migration under flow conditions
• Co-cultivation of cell layers and transport in 2D or in a 3D gel matrix
• Apical-basal cell polarity assays
• Skin and lung models with liquid-air interface
• Cell barrier model assays with apical-basal gradients
• Cell migration assays based on filters and porous membranes

Effortlessly collect 3D topography data with sub-nanometer precision on your benchtop with the Redux AFM. Get quantitative data in minutes for topography, roughness, film thickness, particle size and more.