With L-glutamine Leibovitz’s L15 medium was originally developed without a sodium bicaronate buffer system for use in non-CO2 equilibrated environments.  It is buffered by phosphates and the amino acid L-arginine, along with L-histidine and L-cysteine.  The formation of acid metabolite byproducts is prevented by replacing glucose with galactose and sodium pyruvate.  Examples of usage include the propagation of HEP-2, monkey kidney cells and primary explants of embryonic and adult human tissue.  Leibovitz’s L-15 supports established cell lines as well as primary explants of embryonic and adult human tissue.

With Corning® glutagro™ SupplementCorning hybrigro SF is a complete, animal component-free, defined medium specifically developed for serum-free growth and antibody production with a variety of hybridoma cell lines. -Superior growth and antibody production over serum supplemented media. -Formulated with glutagro to provide a stable, ready to use formulation. No glutamine supplementation required. -Contains Polaxamer 188 to provide shear force protection during agitated suspension culture. -Formulated without phenol red. -Contains low concentrations of highly purified non-animal origin recombinant proteins added for enhanced cell growth and productivity. -Defined: There are no undefined hydrolysates, ultra filtered lysates, or peptides in the formulation.