Corning – Media – Serum Free and Specialty Media

With Corning® glutagro™ SupplementCorning hybrigro SF is a complete, animal component-free, defined medium specifically developed for serum-free growth and antibody production with a variety of hybridoma cell lines. -Superior growth and antibody production over serum supplemented media. -Formulated with glutagro to provide a stable, ready to use formulation. No glutamine supplementation required. -Contains Polaxamer 188 to provide shear force protection during agitated suspension culture. -Formulated without phenol red. -Contains low concentrations of highly purified non-animal origin recombinant proteins added for enhanced cell growth and productivity. -Defined: There are no undefined hydrolysates, ultra filtered lysates, or peptides in the formulation.

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Overview

Features

Specifications

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

Key Features

  • Enhanced Cell Growth and Viability: Formulated to support superior growth and long-term viability of various cell lines, including hybridomas and Chinese Hamster Ovary (CHO) cells.
  • Animal Component-Free: Composed without animal-derived components, reducing the risk of contamination and ensuring consistency across batches.
  • Ready-to-Use Formulation: Supplied as a complete, ready-to-use medium, eliminating the need for additional supplementation and streamlining the cell culture process.

Available Products

Corning® SF Medium

SF Medium is based on a proprietary classical media formulation. This medium contains a mixture of select trace elements and high molecular weight carbohydrates, extra vitamins, animal-free protein, and a small amount of high quality bovine serum albumin (1.0 g/L). SF Medium supports superior growth and viability over long-term passages in hybridomas, as well as suspension and adherent cell cultures. This formulation contains L-glutamine.

Technical Specifications

Product NumberProduct NameQty./PkQty./CsBrandUnit Size
40-101-CVCorning® 500 mL SF Medium, [+] L-glutamine and 1 g/L BSA6 / Pk 6 / Cs Corning® per case
40-102-CVCorning® 500 mL SF Medium [+] L-glutamine, 1 g/L BSA, [-] phenol red6 / Pk 6 / Cs Corning® per case

Corning® Polysucrose 400, Powder

Technical Specifications

Product NumberProduct NameQty./PkQty./CsBrandUnit Size
61-196-RMCorning® 100 g Polysucrose 400, Powder1 / Pk 1 / Ea Corning® per each
61-196-ROCorning® 500 g Polysucrose 400, Powder1 / Pk 1 / Ea Corning® per each

Corning® Lymphocyte Serum-free Medium

Corning’s line of specialty media is optimized for those who require media for a wide range of specialized applications and cell lines.

Technical Specifications

Product NumberProduct NameQty./PkQty./CsBrandUnit Size
88-551-CMCorning® Lymphocyte Serum-free Medium, KBM 5511 / Pk 1 / Cs Corning® per each
88-581-CMCorning® Lymphocyte Serum-free Medium, KBM 5811 / Pk 1 / Cs Corning® per each

Corning® LSM (Lymphocyte Separation Medium)

Lymphocyte Separation Medium was originally designed for the in vitro isolation of lymphocytes from diluted whole blood. It is a sterile filtered, iso-osmotic polysucrose and diatrizoate solution with low viscosity and a density of 1.077-1.080 g/cm³ at 20°C.

Technical Specifications

Product NumberProduct NameQty./PkQty./CsBrandUnit Size
25-072-CICorning® 100 mL LSM (Lymphocyte Separation Medium)1 / Pk 1 / Ea Corning® per each
25-072-CVCorning® 500 mL LSM (Lymphocyte Separation Medium)1 / Pk 1 / Ea Corning® per each

Corning® 500 mL transfectagro™ Reduced-serum Medium, [+] HEPES, glutagro™, and sodium pyruvate

For reduced serum culture conditions. Corning transfectagro Reduced Serum Medium is designed to maximize the growth of a variety of cell types under reduced serum conditions, generally allowing for at least a 50% reduction in serum use with minimal adaptation. transfectagro has also been proven to improve transient transfection efficiency in a variety of cell types. This is a chemically-defined, reduced serum formulation of Eagle’s Minimum Essential Medium for greater buffering capacity.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

Technical Specifications

Product NumberProduct NameQty./PkQty./CsBrandUnit Size
40-300-CVCorning® 500 mL transfectagro™ Reduced-serum Medium, [+] HEPES, glutagro™, and sodium pyruvate6 / Pk6 / CsCorning® per case

Corning® 500 mL Insectagro® Sf9 Serum-Free/Protein-Free Medium, 1X [+] L-glutamine

Corning insectagro Sf9 is formulated to support the propagation of Sf9 insect cells in culture, and can also be used for SF21 cells. The Sf9 cell line originated at the USDA Insect Pathology Laboratory from IPLBSF-21 cell line, derived from the pupal ovarian tissue of the fall army worm. Sf9 cells are cultured in non-humidified, non-CO2 incubators at 27ºC (room temperature) and display both monolayer and suspension culture qualities. With their fast doubling times of 10-22 hours, Sf9 cells are easily scaled up to large cultures using bioreactors. Sf9 is capable of expressing full-length proteins and have been reported to produce certain proteins up to 20% of the total protein.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

Technical Specifications

Product NumberProduct NameQty./PkQty./CsBrandUnit Size
13-410-CVCorning® 500 mL Insectagro® Sf9 Serum-Free/Protein-Free Medium, 1X [+] L-glutamine6 / Pk6 / CsCorning® per case

Corning® 500 mL PF Medium [-] L-glutamine

Optimized for the cultivation of Chinese Hamster Ovary (CHO) cells, along with many derivatives of this parent line, Corning PF Medium is a proprietary serum-free and protein-free growth medium that does not contain any hormones or growth factors.  This medium supports superior growth and viability over long term passages in both adherent and suspension culture.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

Technical Specifications

Product NumberProduct NameQty./PkQty./CsBrandUnit Size
40-200-CVCorning® 500 mL PF Medium [-] L-glutamine6 / Pk6 / CsCorning® per case

Corning® 500 mL hybrigro SF™

With Corning® glutagro™ SupplementCorning hybrigro SF is a complete, animal component-free, defined medium specifically developed for serum-free growth and antibody production with a variety of hybridoma cell lines. -Superior growth and antibody production over serum supplemented media. -Formulated with glutagro to provide a stable, ready to use formulation. No glutamine supplementation required. -Contains Polaxamer 188 to provide shear force protection during agitated suspension culture. -Formulated without phenol red. -Contains low concentrations of highly purified non-animal origin recombinant proteins added for enhanced cell growth and productivity. -Defined: There are no undefined hydrolysates, ultra filtered lysates, or peptides in the formulation.  

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

Technical Specifications

Product NumberProduct NameQty./PkQty./CsBrandUnit Size
40-215-CVCorning® 500 mL hybrigro SF™6 / Pk6 / CsCorning® per case

Corning® 500 mL Insectagro® DS2 Serum-Free/Protein-Free Medium, 1X [-] L-glutamine

Corning Insectagro DS2 Serum-Free/Protein-Free Medium was developed for the growth and maintenance of Drosophila Schneider 2 (S2) cells to be used in heterologous protein expression.  At the optimal temperature range 22-24ºC, DS2 cells grow as a loose monolayer and are readily adaptable to growth in suspension.  Under these conditions, the cells require minimal adaptation to serum-free culture.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

Technical Specifications

Product NumberProduct NameQty./PkQty./CsBrandUnit Size
13-402-CVCorning® 500 mL Insectagro® DS2 Serum-Free/Protein-Free Medium, 1X [-] L-glutamine6 / Pk6 / CsCorning® per case

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