Corning – 3D Clear Tissue Clearing Reagent
Despite the growing relevance of 3D cell culture as a research model, imaging techniques used to characterize these models are highly limited. Due to the thickness and opacity of 3D cellular structures, most current imaging tech…
Despite the growing relevance of 3D cell culture as a research model, imaging techniques used to characterize these models are highly limited. Due to the thickness and opacity of 3D cellular structures, most current imaging technologies cannot penetrate to the center of the tissues, resulting in only the outer 2 to 3 layers of cells being detected. This causes the dark centers often seen in images of 3D cell culture models, which proves highly problematic for accurate analysis as these outer cells are most exposed to compounds, nutrients and oxygen and thus do not reflect the entire cell population.