NanoCellect – N1 Single-Cell Dispenser
The N1 Single-Cell Dispenser is an accessory to the WOLF® G2 Cell Sorter
The system uses aerosol-free, sterile, and disposable microfluidic cartridges. It allows for bulk cell selection and sorting directly into 96-well or 384-well plates. The N1 Single-Cell Dispenser seamlessly integrates into the intuitive software, enabling easy selection of single cells from a dropdown menu, plate mapping, and sorting initiation. The WOLF G2 and N1 dispenser collaborate to efficiently sort and plate cells in a compact and cost-effective setup. Post-sorting, cell data are indexed well-by-well for accurate cell confirmation.
This setup supports bulk sorting for collecting entire populations or individual cell sorting into standard 96- or 384-well plates using the N1 Single-Cell Dispenser module. It’s ideal for cloning sorted cells or conducting plate-based assays. The gentle sorting mechanism enhances cell viability and outgrowth while minimizing cell stress, thereby avoiding potential gene expression or karyotype changes associated with traditional sorters.
Technical Specifications
| Quantity | EA |
| Throughput | Time to plate (96 wells) 3-8 minutes Time to plate (384 wells) 32 minutes |
| Sample Volume | 3 – 10 µL (droplet volume) |
| Sample Type | Single cells such as B-cells, Monoclonal CHO cell line |
| Plate Formats | 96-well or 384 well plates |
Key Features
High Viability
- Ideal for single cell cloning and compatible with the microfluidic-based WOLF cell sorter
- Facilitates seamless single cell isolation, analysis, and cell line development
- Utilizes a gentle low-pressure sorting mechanism and single cell manipulation
- Ensures high outgrowth of healthy clones by preserving cell viability throughout the sorting process
- Minimizes stress with a gentle microfluidic cell sorting process
- Avoids potential adverse gene expression or karyotype changes associated with traditional cell sorters
Sterile Microfluidics
- Uses a minimal fraction of sheath fluid compared to traditional sorters
- Allows for the use of growth media as sheath fluid
- Improves cell viability during cell isolation, FACS, and flow cytometry analysis
Single Cell Efficiency
- Provides 5 parameters for analyzing and sorting cells
- When used with the N1 Single-Cell Dispenser accessory, achieves higher rates of single cell detection in wells.
- Routine deposition rates above 90-95% are observed, surpassing those of cell printers or limited dilution methods
Easy-to-use
- No expertise in flow cytometry required to operate the WOLF
- Intuitive and simple software interface makes it easy to use
- Eliminates the need for a fluidics cart and features fixed optics
- Reduces complexity, cleanup, and allows more time for experiments
Small Footprint
- Unlike traditional flow cytometry setups, the robust, portable, sterile, and safe benchtop system can operate in any lab environment
- No need for dedicated hoods, venting systems, or specialized personnel
- Lightweight design allows for easy mobility and sharing between labs
- Fits comfortably within a biosafety cabinet, accommodating both the WOLF and the N1 single-cell dispenser
Discover the Power of the N1 Single-Cell Dispenser
Welcome to an in-depth look at the N1 Single-Cell Dispenser by NanoCellect. In this video, we showcase how the N1 enhances your cell sorting and dispensing workflows, making single-cell research more efficient and precise than ever before.
NanoCellect is committed to empowering every scientist to make discoveries one cell at a time, with modern and simple technologies…
