Malvern Panalytical – Microcal Range – Microcalorimeters
Microcalorimeters for characterization of biomolecular stability and interactions
MicroCal microcalorimeters are powerful, analytical tools used to study biological systems. These include interactions between molecules as well as changes in conformation such as protein unfolding. The technique is very convenient requiring only minimal assay development and NO labelling. The range includes both Differential Scanning and Isothermal Titration calorimetry.
MicroCal microcalorimeters are used in a wide range of applications from basic research to the discovery and development of small molecule drugs, biotherapeutics and vaccines. Our customers include pharmaceutical, biotech, academic and governmental research institutions worldwide.
Available Models
Technical Specifications
| Measurement | Enthalpy,Affinity,Entropy,Stoichiometry |
| Temperature range | 2°C to 80°C |
|---|---|
| Temperature stability | ± 0.00012°C |
| Sample capacity | 384 (four 96 well plates) |
| Sample volume | 370 µL |
| Cell volume | 200 µL |
| Injection syringe volume | 40 µL |
| Injection volume precision | <1% @ 2µL |
| Throughput | Up to 42 per 24 hr |
| Cell material | Hastelloy |
| Noise | 0.15 ncal/s |
| Response time | 8 s |
High Productivity Measurement of Multiple Binding Parameters
The MicroCal PEAQ-ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter which offers the convenience of unattended operation. It delivers direct, label-free in solution measurement of all binding parameters in a single experiment. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. It can also be used to measure enzyme kinetics
Technical Specifications
| Measurement | Enthalpy,Affinity,Entropy,Stoichiometry |
| Temperature range | 2°C to 80°C |
|---|---|
| Temperature stability | ± 0.00012°C |
| Sample capacity | 384 (four 96 well plates) |
| Sample volume | 280 µL |
| Cell volume | 200 µL |
| Injection syringe volume | 40 µL |
| Injection volume precision | <1% @ 2µL |
| Throughput | 8-12 per 8 hr |
| Cell material | Hastelloy |
| Noise | 0.15 ncal/s |
| Response time | 8 s |
High Sensitivity Measurement of All Binding Parameters
The MicroCal PEAQ-ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter which offers the convenience of unattended operation. It delivers direct, label-free in solution measurement of all binding parameters in a single experiment. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. It can also be used to measure enzyme kinetics
Technical Specifications
| Sample capacity | 288 (6 × 96-well plates) |
| Sample volume | 325 µL |
| Typical sample concentration | 0.01 mg/mL – 10 mg/mL 1 |
| Sample throughput | ≤50 samples/day |
| Sample storage temperature range | 4°C – 40°C |
| Operating temperature (°C) | #ERROR! |
| Storage temperature | -20°C to +50°C |
| Humidity | 10% to 70%, non-condensing (10% to 90% for storage) |
| Ingress Protection (IP) rating | IP21 |
| Power | 100-240 V A/C, 50/60 Hz, 70 W (cell), 400 W (max, autosampler), PC as supplied |
| Certification | CE (EN61010-1), EMC (EN61326-2-1, EN61326-1, FCC, ICES, VCCI), ISO9001:2008 |
| Dimensions (W x D x H) | 101 cm × 68 cm × 70 cm |
| Weight | Approx. 25 kg |
Gold Standard Protein Stability Analysis for the Regulated Environment
The MicroCal PEAQ-DSC Automated system delivers high throughput, highly sensitive protein stability analysis with low sample consumption, in an automated, integrated platform for increased productivity. All cell filling and cleaning functions are fully automated for walk-away operation.
Key Features
- Gold standard stability-indicating technique requiring minimal assay development
- Direct and label-free measurement of biomolecular native-state stability in solution
- Standard 96-well plate format for high capacity and easy loading, with thermostatically-controlled storage of up to 6 plates
- Fully integrated autosampler enables unattended analysis of up to 50 samples per day
- Automated cell filling and cell cleaning functions permit unattended operation
- Measurement of very tight binding constants, up to 1020M-1
- Powerful MicroCal PEAQ-DSC software minimizes typical data analysis time
- PEAQ-Compliance: The system is available with Malvern Access Controller (MAC) to limit access to user-defined SOPs and data analysis features. Data can be displayed using the Report Generator with user details and the ability to sign using electronic signatures to facilitate the integration of the workflow into your company’s quality system and aid compliance with 21 CFR Part 11 and Annex 11 regulations. This is provided as an optional accessory
- PEAQ-Performance: Automatically detects and validates system readiness for optimal performance
- PEAQ-Smart (including PEAQ-Finder): For SOP-based operation and data analysis. Provides new algorithms to detect even subtle peaks and shoulders, facilitating the automatic, non-subjective identification of multiple transitions, such as those seen in multi-domain proteins
- PEAQ-Compare: For the quantitative comparison of DSC traces for comparability studies, ideal for batch-to-batch and biosimilarity studies
- Network-ready: email updates sent during the analysis to keep you informed of assay progress
Technical Specifications
| Sample volume | 250 µL (manual filling) |
| Typical sample concentration | 0.01 mg/mL – 10 mg/mL 1 |
|---|---|
| Sample throughput | ≤6 samples analyzed/8h |
| Operating temperature (°C) | +10°C to +28°C |
| Storage temperature | -20°C to +50°C |
| Humidity | 10% to 70%, non-condensing (10% to 90% for storage) |
| Ingress Protection (IP) rating | IP21 |
| Power | 100-240 V A/C, 50/60 Hz, 70 W (cell), PC as supplied |
| Certification | CE (EN61010-1), EMC (EN61326-2-1, EN61326-1, FCC, ICES, VCCI), ISO9001:2008 |
| Dimensions (W x D x H) | 20 cm × 44 cm × 19 cm |
| Weight | 8.2 kg |
| 1 Sample dependent |
Gold Standard Protein Stability Analysis for Research Applications
The MicroCal PEAQ-DSC system provides highly sensitive, easy-to-use microcalorimetry that helps reduce the time and cost associated with stability testing and comparability analysis. The MicroCal PEAQ-DSC is a manual instrument with a cleaning device, which can be upgraded to an automated version upon request.
Key Features
- Gold standard stability-indicating technique requiring minimal assay development
- Direct and label-free measurement of biomolecular native-state stability in solution
- Measurement of very tight binding constants, up to 1020M-1
- Manual system with pipette and cleaning device
- Powerful MicroCal PEAQ-DSC software minimizes typical data analysis time
- PEAQ-Compliance: The system is available with Malvern Access Controller (MAC) to limit access to user-defined SOPs and data analysis features. Data can be displayed using the Report Generator with user details and the ability to sign using electronic signatures to facilitate the integration of the workflow into your company’s quality system and aid compliance with 21 CFR Part 11 and Annex 11 regulations. This is provided as an optional accessory
- PEAQ-Performance: Automatically detects and validates system readiness for optimal performance
- PEAQ-Smart (including PEAQ-Finder): For SOP-based operation and data analysis. Provides new algorithms to detect even subtle peaks and shoulders, facilitating the automatic, non-subjective identification of multiple transitions, such as those seen in multi-domain proteins
- PEAQ-Compare: For the quantitative comparison of DSC traces for comparability studies, ideal for batch-to-batch and biosimilarity studies
- Network-ready: email updates sent during the analysis to keep you informed of assay progress
Key Features
- Measurements of binding affinity and thermodynamic parameters in a single experiment
- High sensitivity with options for automation to minimize ‘hands on’ time
- Low sample consumption
What Is Differential Scanning Calorimetry (DSC)?
Malvern Instruments MicroCal DSC team introduce what Differential Scanning Calorimetry (DSC) is and why it is the gold standard in characterizing the stability of a protein or other biomolecules.
The Malvern MicroCal team details how Differential Scanning Calorimetry (DSC) works and why you should be using it for testing protein stability.
Key Industries
- Biopharmaceutical and Biotechnology
- Chemical
- Medical
- Pharmaceutical
Malvern Panalytical was formed by the merger of Malvern Instruments Limited and Panalytical B.V. on 1st January 2017, has headquarters…
