Biochrom – Amino Acid Analyzer – 30+ series

Technical Specifications

Detection System	Nninhydrin – post column derivatization
Reproducibility	Area: Better than 0.5% RSD at 10 nanomoles. Retention time: Better than 0.1%RSD
Pumps	Two dual piston pumps with integrated pressure transducer and drain valve. Ceramic pump head, sapphire pistons and valves. Electronically controlled piston wash.
Operating Pressures	Buffer pressure: maximum 150 bar. Ninhydrin pressure: maximum 24 bar
Analytical Column	High pressure PEEK column packed with Ultropac 8 cation exchange resin. Peltier heating/cooling system.
Sample Injection	3 injection modes (full loop, partial loop and micro), 84 position autosampler. Sample volumes from 1μL to 5000μL. 200μL loop supplied as standard.
Temperatures	Column temperature variable between 20°C and 99°C. Reaction Coil temperature adjustable between 40°C and 145°C (135°C is optimum).
Software	BioSys v3.0 control software Biochrom Alias Manager autosampler control software Latest version of EZChrom Elite Data Handling 21 CFR part 11 compliant
Photometer	Single flow cell with optical beam splitter with detection at 440nm and 570nm
Weight and dimensions	Bench top Fluidics Cabinet: 50kg/110lbs, 48 x 59 x 57cm /19 x 23 x 22 inches Autosampler: 21kg/46lbs, 30 x 57.5 x 36cm /12 x 23 x 14in (cooled version) 19kg/42lbs, 30 x 51 x 36cm/12 x 20 x 14in (ambient version)
Required Services	Oxygen free Nitrogen gas (99.99%) or Argon gas regulated to 5 bar. Drainage facility. 240V/100V, 50Hz/60Hz, 300VA mains supply.
Safety Systems	Automatic shut-down and reaction coil flushing in the event of: Power failure Photometer lamp failure Low ninhydrin, buffer, coil and nitrogen pressures High coil and column temperatures, high ninhydrin, buffer and coil pressures.

Key Features

• Accurate: 
  Superb separation, accurately identifies and quantifies free amino acids, as well as the amino acid composition of proteins and peptides.
• Reliable:
  Minimal matrix interference and near baseline separation helps to reduce ambiguities in interpretation and increase confidence in diagnosis of inborn errors of metabolism. Automated derivatization of amino acids maximizes reproducibility.
• Fast:
  Rapid protocols that increase sample throughput by cutting analysis time.
• Economical: 
  The column cleaning and repacking service, gives columns an almost indefinite life and cuts the cost of ownership.
• Fully Automated:
  Total PC control allows 24/7 operations.
• Versatile:
  Wide variety of sample types compatible: Urine, plasma, serum, CSF, amniotic fluid, culture media, tissue extracts, protein hydrolysates from various food and feedstuff samples or synthetic drugs, nutraceuticals, beverages.
• Easy to implement: 
  No lengthy method development required as this well characterized system is established worldwide. All customers have their instrument optimised to their application directly from the factory.
• Convenient:
  Interchangeable sample trays allow samples to be prepared in advance and stored refrigerated.
• Robust: 
  Advanced fluidics and robust hardware mean easy maintenance for users, backed by the reassurance of Biochrom’s dedicated service and support team.
• Flexible: 
  Integrated software, choice of different methods to adapt to the user’s needs. Automatic sample set-up. Networking and exporting of data.
• Compact: 
  Bench top design requiring minimum lab space.

Biochrom 30 Amino Acid Analyzer