Ibidi – Labware – ยต-Slide Spheroid Perfusion
A Perfusable Channel ยต-Slide With 3 x 7 Wells for Long-Term Spheroid Cultivation and High-End Microscopy
Product outline
- Ideal for the long-term cultivation and perfusion of 3D spheroids or organoids on the Bioinert surface
- Flexible preparation for perfusion: spheroids can be transferred or created directly in the wells
- Imaging chamber with excellent optical quality for high-resolution microscopy
Applications
- Cultivation and high-resolution microscopy of three-dimensional cell aggregates (e.g., spheroids and organoids)
- For use with the ibidi Pump System or any other pump device with Luer connectors
- Perfusion of spheroids for fresh medium supply during long-term cultivation and access to spheroid growth kinetics
- Spheroid generation directly in the wells
- Spheroid retrieval for downstream processing (e.g., immunofluorescence, histology, and biochemical assays)
- Perfusion of cell monolayers (adherent cells), suspension cells, or co-cultures
- Organ-on-a-chip setups with up- and downstream metabolization
- Live cell imaging and microscopy of 3D cell aggregates
- Immunofluorescence staining and high-resolution fluorescence microscopy of living and fixed cells and cell aggregates
Technical Specifications
| Outer dimensions (w x l) | 25.5 x 75.5 mmยฒ |
| Number of channels | 3 |
|---|---|
| Number of wells | 3 x 7 |
| Volume per well | 3.5 ฮผl |
| Well height (bottom niche) | 0.4 mm |
| Well height (total) | 1.3 mm |
| Well diameter (bottom) | 0.8 mm |
| Channel volume (total) | 45 ยตl |
| Channel height | 0.2 mm |
| Channel width | 1.0 mm |
| Growth area per well | 0.5 mmยฒ |
| Coating area using 3.5 ยตl | 9.7 mmยฒ |
| Adapters | Female Luer |
| Volume per reservoir | 60 ยตl |
Slide Comparison
| ยต-Slide Spheroid Perfusion | ยต-Slide I Luer 3D | ยต-Slide I Luer | |
| Application | |||
| Perfusion of samples | Yes | Yes | Yes |
| Defined shear stress on cell monolayers | No | Yes, on gel matrix | Yes, on coverslip |
| Gel matrices for 3D | No | Yes | No |
| Cell type | |||
| Spheroids/organoids | Yes, free floating in well | Yes, inside gel matrix only | No |
| Suspension cells | Yes, free floating in well | Yes, inside gel matrix only | Yes, in flow suspension only |
| Adherent cells | Yes, on coverslip | Yes, inside or on gel matrix | Yes, on coverslip |
Brand
ibidi is a leading supplier for functional cell-based assays and advanced products for cellular microscopy. ibidi is located in Grรคfelfing, Germany, close to Munich, and the US headquarters, ibidi USA Inc., is located in Fitchburg, Wisconsin.
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