Thermo Scientific™ – Food Authenticity – PCR Testing for Meat Species
Meat Species Detection, Screening and Quantification
Imegen™ RapidFinder™ Meat ID Detection Kits have been developed in collaboration with Imegen to create a broad portfolio of real-time PCR-based animal species detection and quantification solutions. Among others, the range includes assay kits for beef, poultry, ruminant, equine and pork DNA in food and feed samples. The assays provide very high sensitivity with a limit of detection (LOD) below the officially required level of 0.1%.
Available Models
Technical Specifications
| Includes | 1 box |
| Quantity | Contains 1 tube each of: Vegan Master-Mix (-20°C), Vegan Positive Control (-20°C), General Master-mix (4°C) |
| Shelf life | 365 days |
| Detection method | Fluorescence |
| For use with (Application) | RT-PCR |
| GC-Rich PCR performance | High |
| No. of reactions | 48 |
| PCR method | RT-PCR |
| Product type specs | Vegan identification kit |
| Reaction speed | Fast |
| Unit size | Each |
The RapidFinder™ Vegan ID Kit uses real-time PCR to target a mitochondrial DNA region which is conserved in all animal species, providing highly sensitive detection of any animal species. The kit supports food laboratories to check the vegan status of food products and ingredients as any item of animal origin is detected including meat, fish, eggs and dairy.
How the RapidFinder™ Vegan ID Kit Works
Animal DNA detection is accomplished by real-time PCR using two TaqMan™-MGB probes. The target sequence, detected in the FAM dye channel, specifically detects one mitochondrial DNA sequence common to all animal species. Also included in each reaction mix is an internal positive control detected in the VIC™ dye channel, which is used to verify the absence of PCR inhibitors.
By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% bovine DNA, and by comparing sample results to the control results, a threshold of detection is provided.
Sample Preparation
DNA sample preparation for the RapidFinder™ Vegan ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed foods, as well as composite food samples.
Part of a Suite of Solutions for Meat Species Detection
The RapidFinder™ Vegan ID Kit is just one of our many kits available for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of pork, horse, sheep, ruminant, chicken, turkey, and poultry.
Technical Specifications
| Includes | 1 box |
| Quantity | Contains 1 tube each of: Halal Master-Mix (-20° C), Halal Positive Control (-20°C), General Master-mix (4°C) |
| Shelf life | 365 days |
| Detection method | Fluorescence |
| For use with (Application) | RT-PCR |
| GC-Rich PCR performance | High |
| No. of reactions | 48 |
| PCR method | RT-PCR |
| Product type specs | RT-PCR kit |
| Reaction speed | Fast |
| Unit size | Each |
Support Halal label claims by testing for the presence of pork DNA in food and feed samples to verify if any adulteration or cross-contamination has occurred in purchased ingredients or during manufacture.
How the RapidFinder™ Halal ID Kit Works
Animal DNA detection and identification is accomplished by real-time PCR using two TaqMan™-MGB probes. The target sequence, detected in the FAM dye channel, specifically detects a mitochondrial DNA sequence specific for Sus scrofa (pork). Also included in each reaction mix is an internal positive control detected in the VIC® dye channel, which is used to verify the absence of PCR inhibitors.
By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.0005% pork DNA, and by comparing sample results to the control results, a threshold of detection is provided.
Sample Preparation
DNA sample preparation for the RapidFinder™ Halal ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample, rather than the smaller sample sizes accommodated by most other DNA isolation procedures, minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.
Part of a Suite of Solutions for Meat Species Detection
The RapidFinder™ Halal ID Kit is just one of our many kits available for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of beef, horse, sheep, ruminant, chicken, turkey, and poultry.
Technical Specifications
| Kit type | Equine ID kit |
| No. of reactions | 48 reactions |
| Product line | RapidFinder™ |
| Equine Master Mix | Store at -5 to -30°C |
| General Master Mix | Store at 2–8°C |
| Positive Control | Store at -5 to -30°C |
The RapidFinder™ Equine ID Kit enables the simple and reliable detection of horse meat in food and feed samples. The method works by detection of horse (Equus caballus) DNA by real-time PCR. Unlike ELISA based methods, the RapidFinder™ Equine ID Kit detects both raw and processed horse meat and provides sensitivity down to 0.01% horse meat DNA when used with the GMO Extraction Kit. A positive control is included in the kit (0.1% equine DNA) that can be used as a reference to set the threshold of detection of the assay.
How the RapidFinder™ Equine Kit Works
Equine DNA detection is accomplished by real-time PCR using the supplied primer/probe set specific for a mitochondrial DNA sequence that is unique to Equus caballus. The target sequence, detected in the FAM™ channel, is not only specific for horse, but also stable. Included in each reaction mix is an internal positive control detected in the VIC® channel, used to verify the absence of PCR inhibitors.
By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% horse DNA in a background of beef DNA, and by comparing sample results to control results, a threshold of detection is provided.
The assay is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.
Sample Preparation
DNA sample preparation for the RapidFinder™ Equine ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit. Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.
Key Features
PCR Testing for Meat Species
- Simple: real-time PCR based assay, no electrophoresis needed
- Flexible: animal DNA detected in any food or feed sample, both raw and processed
- Reliable: Internal Positive Control (IPC) provides process-control check for PCR inhibitors
- Fast: from sample to result in the same day
Key Industries
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Brand
Thermo Fisher Scientific is the world leader in serving science, with revenues of more than $24 billion and approximately 70,000 employees globally. Our mission is to enable our customers to make the world healthier, cleaner and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics, deliver medicines to market and increase laboratory productivity. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services – we offer an unmatched combination of innovative technologies, purchasing convenience and comprehensive services.
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