Thermo Fisher – Solvents and Blends for HPLC Applications
Our chromatography solvents are made specifically for HPLC instrumentation. They are prepared by a distillation process to remove volatile impurities that can contaminate the analytical columns and reduce their lifetimes, followed by filtration to achieve maximum purity. These solvents have low UV absorption and low peak interference and are free of particles up to 0.02 micron. Thus, usage of our high purity HPLC solvents extends column lifetime. Our HPLC grade solvents are also designed to significantly reduce detector noise and baseline drifts, thereby enhancing data quality and helping ensure reproducibility.
Available Models
Improving Separation in Reversed Phase HPLC
High performance liquid chromatography
Application Note
Separation in reversed phase liquid chromatography depends on non-polar analytes being retained by a hydrophobic stationary phase such as a C18 column. If the sample contains ionic (polar) components, they may not be sufficiently retained by the stationary phase for adequate separation. The primary methods of improving retention are via ion pairing or pH modification.
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