PacBio – Long-read Sequencing – Revio System


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  • Long-read Sequencing – Revio System brochure – PacBio

  • PacBio – Long-read Sequencing – Revio System Spec Sheet

The Long-read Sequencing – Revio System adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

The Long-read Sequencing – Revio System empowers HiFi sequencing. It adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

Revio features advanced sequencing consumables, robotics, and massive compute power to deliver accurate long reads with methylation information directly from the sequencer in every run. Free yourself to discover more with a complete view of genomes, epigenomes, and transcriptomes on the Revio system.

Technical Specifications

Temperature19–25°C (66–77°F)
Humidity20–80% relative humidity, non-condensing
Ventilation13,000 BTU/hr (3,800 W)
Dimension (W x D x H)92.7 cm (36.5 in) × 91.4 cm (36.0 in) × 174.5 cm (68.7 in)
Weight465 kg (1,025 lb)
Floor Space243.8 cm (96.0 in) × 138.5 cm (54.5 in)
Power requirements200–240 VAC at 50-60 Hz, max 5 kVA/4.8 kW/24A
Network connection1 GbE or 10 GbE, copper
Output file formathifi_reads.bam, ≈55 GB each, up to 72 TB per year

Key Features

With a high-density, 25 million ZMW SMRT Cell, up to 4 SMRT Cells in parallel, and 24-hour run times, the Revio system delivers 360 Gb of HiFi reads per day, equivalent to 1,300 human whole genomes per year. 

HiFi sequencing provides small variants, structural variants, repeat expansions, methylation, and haplotype phasing from a single library and sequencing run. ​

Operating the Revio system is simple, thanks to a combined reagent and sample plate, rapid run setup, flexibility to queue runs while sequencing is in progress, and no external nitrogen supply. ​

Advanced algorithms on the Revio system deliver exceptional read accuracy plus methylation calling in every run. Its optimized file formats reduce data storage needs through quality value binning and smart read ordering.

Unleash the Power of HiFi Sequencing

Get Your Own Revio System at a Special Price

It’s now easier than ever to detect structural variants, repeat expansions, and methylation patterns with extraordinary accuracy and high throughput.

The PacBio Revio system delivers HiFi long-read sequencing at scale adding accuracy, low cost, and ease of use to the other benefits of long reads.



PacBio offers revolutionary sequencing technologies that combine the completeness of long reads with the accuracy of short reads to provide the most comprehensive view of genomes, transcriptomes, and epigenomes.

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