Pacbio – Short-read Sequencing – Onso System


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  • Short-read Sequencing – Onso System brochure – PacBio

  • Short-read Sequencing – Onso System spec sheet – PacBio

The Pacbio – Short-read Sequencing – Onso System is an innovative benchtop short-read DNA sequencing platform with an extraordinary level of accuracy using PacBio sequencing by binding (SBB) technology. Ultra-high Q40+ data quality allows you to break through limits of detection.

The Onso short-read sequencing system is a scalable and flexible benchtop platform that provides an extraordinary level of accuracy through its unique sequencing by binding (SBB™) technology. With inherently lower sequencing noise, the Onso system enables researchers to uncover rare variants previously missed and do so at lower sequencing depths.

The accuracy provided by the Onso system will help drive groundbreaking discoveries in areas such as liquid biopsy testing for minimal residual disease (MRD), antibiotic resistance monitoring in microbial communities, off-target analysis in gene editing experiments, and many other “needle-in-a-haystack” applications where the highest level of sensitivity is desired.

Sequencing Specifications

Onso reagentsRead lengthReadsOutput (GB)Run timeQuality score
200 cycle sequencing kit2×100 bp
1×200 bp
400—500 M80—10032 hours≥90% Q40
300 cycle sequencing kit2×150 bp400—500 M120—15048 hours≥90% Q40

Technical Specifications

Temperature18–26°C (64–79°F)
Humidity20–80% relative humidity, non-condensing
AltitudeBelow 2,000 m (6,562 ft)
Sequencer dimension (W x D x H)37 in (94 cm) × 29 in (73.7 cm) × 25.75 in (65.4 cm)
Weight133.8 kg (295 lb)
Cluster generator dimension (W x D x H)32 in (81.3 cm) x 22 in (55,9 cm) x 26 in (66 cm)
Weight43.5 kg (96 lb)
Power requirements100–240 V at 50–60 Hz
Network connection≥10 Gbps
ICC operating systemWindows 10 Enterprise
Output file formatFASTQ
Output file size35–60 GB

Key Features

SBB chemistry to deliver ultra-high quality, highly accurate, and exceptionally sensitive detection of cfDNAs.

Delivering the ability to identify biomarkers missed by other sequencing platforms.

Less sequencing per sample allows reduced cost and increased throughput.

Designed with key accessories and the flexibility to integrate with existing short-read tools:

  • Library conversion kit
  • PacBio native library prep kits (HMW and fragmented DNA preparation)
  • Blocking oligos for hybridization capture methods
  • Library amplification kit
  • Library quantification kit
  • Lambda sequencing control library (spike-in library)

A Groundbreaking New Sequencer



PacBio offers revolutionary sequencing technologies that combine the completeness of long reads with the accuracy of short reads to provide the most comprehensive view of genomes, transcriptomes, and epigenomes.

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