Ibidi – Labware – ยต-Slide I Luer 3D
A ยต-Slide With One Channel and Three Wells for Culturing Cells on a 3D Gel Matrix With Defined Flow
The ยต-Slide I Luer 3D is designed for culturing cells on a 3D gel matrix under defined flow conditions, simulating in vivo-like environments for endothelial and cell barrier studies.
Key Features
- Create a perfusable cell monolayer on a gel matrix for various flow and shear stress applications
- Establish and image endothelial barriers under in vivo-like conditionsโno artificial filters or membranes
- Easy handling: samples are placed into the open wells; perfusion is applied after closing the wells
Applications
- Culture of adherent cells on a 3D gel matrix under flow conditions
- Transendothelial migration studies under flow conditions
- Establishment and microscopic read-out of endothelial barrier assays (without any artificial membrane)
- Simulation of blood vessels with endothelial cell culture on a soft substrate
- Long-term or short-term cell culture perfusion experiments with defined shear stress and optimal nutrient supply into 3D structures
- Apical-basal cell polarity assays
- Cell barrier model assays with apical-basal gradients
- Cell boundary assays without filters or porous membranes
- Rolling and adhesion assays using leukocytes
- Inflammation studies
- Transport studies
Technical Specifications
| Outer dimensions (w x l) | 25.5 x 75.5 mmยฒ |
| Number of wells | 3 |
|---|---|
| Volume of wells | 16 ฮผl |
| Well dimensions | 5.4 mm x 4.0 mm |
| Well height (without channel) | 0.8 mm |
| Growth area per well | 0.21 cmยฒ |
| Coating area per well | 0.34 cmยฒ |
| Channel width | 5.0 mm |
| Channel volume (without wells) | 150 ฮผl |
| Channel height (without well) | 0.6 mm |
| Adapters | Female Luer |
| Volume per reservoir | 60 ฮผl |
| Top cover | ibidi Polymer Coverslip |
| Bottom | ibidi Polymer Coverslip |
Slide Comparison
| ยต-Slide Spheroid Perfusion | ยต-Slide I Luer 3D | ยต-Slide I Luer | |
| Application | |||
| Perfusion of samples | Yes | Yes | Yes |
| Defined shear stress on cell monolayers | No | Yes, on gel matrix | Yes, on coverslip |
| Gel matrices for 3D | No | Yes | No |
| Cell type | |||
| Spheroids/organoids | Yes, free floating in well | Yes, inside gel matrix only | No |
| Suspension cells | Yes, free floating in well | Yes, inside gel matrix only | Yes, in flow suspension only |
| Adherent cells | Yes, on coverslip | Yes, inside or on gel matrix | Yes, on coverslip |
ibidi is a leading supplier for functional cell-based assays and advanced products for cellular microscopy. ibidi is located in Grรคfelfing,…
