Ibidi – Instruments – ibidi Pump System
A Pump System for the Cultivation of, e.g. Endothelial, Cells Under Flow for Simulation of Blood Vessels
The ibidi Pump System consists of two main components: the ibidi Pump and the Fluidic Unit. The ibidi Pump is an air pressure generator that is computer-controlled and designed to sit outside the incubator.
Key Features
- A pump system for the cultivation of, e.g. endothelial, cells under flow for simulation of blood vessels.
- Ideal simulation of various physiological conditions – continuous unidirectional, oscillating, and pulsatile flow.
- Compatible with the ibidi Heating Systems, all incubators, incubated microscopes, and Channel µ-Slides.
- Minimal mechanical stress, minimal amount of medium and supplement needed.
Technical Features
- Operates up to four parallel Fluidic Units per ibidi Pump
- Flow characteristics: all flow types are laminar
- Some examples:
- Unidirectional flow simulates veins or small arteries
- Oscillating flow simulates disturbed conditions at vessel branching’s or valves
- Pulsatile flow simulates arterial conditions
- Wide range of flow rates (ca. 0.1–40 ml/min*) and shear stress (ca. 0.2–150 dyn/cm2*)
- Working volume: 2.5 ml / 12 ml / 50 ml
- Suitable for all µ-Slides with Luer adapters
- Also suitable for home-made flow chambers
- Compatible with all incubators
- Software-controlled flow rates and shear stress
*Depending on the Perfusion Set and Channel used.
System Overview
Applications
- Defined shear stress in long-term culture (e.g., endothelial cells, kidney, or biofilm)
- Live cell imaging and immunofluorescence for analysing shear stress response
- Mimicking shear stress conditions in microcapillary, venous, and arterial flow
- Rolling and adhesion of suspended cells on substrates.
- Stop flow experiments
- 3D cell culture: interstitial flow
Components of the ibidi Pump System
ibidi Pump
- Computer-controlled air pressure pump
- Operates up to four parallel Fluidic Units per ibidi Pump
PumpControl Software
- Easy setup of an automated flow control for flow assays
- Internal calculation for flow rates, shear rates, and shear stress
Perfusion Set
- Includes tubing and reservoirs to perform flow assays in combination with the Fluidic Unit
- Designed for various flow rates and volumes
Fluidic Unit
- Flow-switching device with electrically controlled valves
- Equipped with a holder for one Perfusion Set (10 ml) and connected µ-Slide(s)
- For operation in the cell culture incubator
- Optional: Reservoir Holder for 2 ml and 50 ml Perfusion Sets
ibidi is a leading supplier for functional cell-based assays and advanced products for cellular microscopy. ibidi is located in Gräfelfing,…
