ATTO – Luminometer
“ATTO Phelios” luminometer offers superior performance for measuring bioluminescence and chemiluminescence in flash and glow assays with high sensitivity and reliability, and it is a useful tool for diverse applications from ELISA to cell based assays.
Since 1980, ATTO has been developing luminescent detectors for HPLC. Our team developed measurement methods for biological activities of living cells by bioluminescence and chemiluminescence, and finally we commercialized microtiter plate reader “Luminescencer” in 1991. In 1997, ATTO released “Luminescencer JNR” as a luminometer for microtiter plates, followed by “Luminescencer PSN” as a luminometer for microtubes. The consistent innovation of ATTO has established it as a luminescence technology leader.
Furthermore, ATTO has developed a variety of basic technologies, including bioluminescent imaging, active oxygen measurement, multicolor separation, luminescent spectrophotometer, secretory luciferase (Cluc), and studied the mechanism of firefly luciferase reaction, global standardization of luminescent measurements, and near infrared measurement.
Available Models
Technical Specifications
| Measurement type | Absorbance (ABS)/Luminescence | |
| Measurement method | Absorbance measurement: Photodiode Luminescence measurement: PMT | |
|---|---|---|
| Measurement mode | Absorbance measurement: â‘ Endpoint â‘¡ Kinetics â‘¢ Spectrum â‘£ Well-area scan Luminescence measurement: â‘ Endpoint â‘¡ Kinetics Microvolume measurement: â‘ Endpoint â‘¢ Spectrum | |
| Detection sensitivity | Absorbance measurement: 0 – 4.0 O.D. Luminescence measurement: 10-18 mole ATP | |
| Measurement plate (Micro plate) | 6 / 12 / 24 / 48 / 96 / 384 well plate | |
| Measurement plate（Nano Volume Plate) | 3 × 8（24 samples） | |
| Size / Weight | 335mm（W）× 305mm（D）× 232mm（H）/ 7.0kg（AC adapter 0.5kg） | |
| View the entire technical specifications in the brochure. | ||
Multimode microplate reader for multi-purpose
A multimode microplate reader measuring absorbance (ABS), kinetics, spectrum, area scan and luminescence (LUMI), with a wavelength range of 200 to 999 nm. It is also capable of measuring microvolume samples (NANO: 2L).
It is a multi-plate format compatible with 6/12/24/48/96/384 well plates. The “quantitative analysis mode” of quantitative analysis automatically establishes calibration curves and converts concentrations during the measurement, and the analysis results can be saved in Excel.
“Qualitative mode” enables users to establish cutoff values and determine whether target molecules are positive or negative. Furthermore, the optional “Nano Volume plate” can be used to measure absorbance and spectral properties of 24 samples in a minimum volume of 2 µL.
Visit the brochure to find out more about the application.
Technical Specifications
| Sample format | 96/384 well plate format | |
| Detector | Photomultiplier (PMT) Photon counting methods | |
|---|---|---|
| Spectral range | 350～670 nm | |
| Filters | F0: no filter, F1: 560nm LP, F2: 600nm LP | |
| Color separation | Up to three luminescence colors can be separated with automatic filter change mechanism | |
| Injectors | Built-in plunger type 96 well: 20 to 250 uL 384 well: 20 to 100 uL Additional pump system (optional, available for only 96 well plate) | |
| Temperature control | Ambient＋5 ºC～40ºC | |
| Counting times | 96 well 1 to 1200 sec, 384 well 1 to 600 sec | |
| Size | 398 (W) × 460 (D) × 286 (H) mm/16.5 kg | |
| Power | 100-240V 50/60Hz 100VA | |
| View the entire technical specifications in the brochure. | ||
Phelios(AB-2350) is a microplate luminometer for measuring multiple samples with high efficiency of light detection. Phelios is available to measure the diverse wavelength of bioluminescence from 350 to 670nm in 96,384 well microplate. It is designed to achieve high sensitivity with an optical system that is extremely desirable for bioluminescent detection in low noise photon counting.
Additionally, it is capable of reproducible measurement by means of an internal temperature controller and an accurate pump in repeated injection. It is also possible to measure the samples by automatic filtering even when different multi-colors are generated.
Key Features
- Wide wavelength range detection between 350-670 nm – Red luminescence can be detected with high sensitivity
- Wide dynamic range (8 logs)
- High sensitivity – Detection limit is 1zmole (10-21 moles) of luciferase molecules.
- Temperature control system (Ambient+5ºC～40ºC) – Offer appropriate condition to measure light from living cells and tissue samples.
- Auto injection system – Programmable injector condition which enable to detect flash-based luminescence
- Color separation system – Tricolor of luminescence can be separated and analyzed automatically with filter system (560LP, 600LP)
- Measuring samples from flash-based light (20 msec) to long period observation (7 days)
Visit the brochure to find out more about the application.
WSL-2300 Measurement Methods and Modes
| Method | Mode | Plate type | ||
|---|---|---|---|---|
| Absorbance measurement(ABS) | Endpoint | 1-6 wavelengths can be set. The absorbance of a selected well is measured once at each wavelength. The measured value is displayed as Optical Density (O.D.). | 384/96/48/24/12/6 | |
| Kinetics | Measured over time, the readings for each time are displayed. The measurement is repeated at set intervals until it’s reached to the set measurement time. | 384/96/48/24/12/6 | ||
| Spectrum | Measure the absorbance spectrum in a set wavelength range of 200 – 999 nm and wavelength interval (2 nm<). | 384/96/48/24/12/6 | ||
| Area scan | Set the scan pattern (3 x 3 to 13 x 13 area) in each well of the microplate and measure the absorbance for each well area | 96/48/24/12/6 | ||
| Luminescent measurement (LUMI) | Endpoint | Measures a luminescence of a specifi ed well once in a set measurement time (10-1000ms). It is displayed in relative luminescence units (RLU). | 384/96/48/24/12/6 | |
| Kinetics | Measured over time, the readings for each time are displayed. The measurement is repeated at set intervals until it’s reached to the set measurement time. | 384/96/48/24/12/6 | ||
| Micro-volume measurement (NANO) | End point | Use 2 – 5μL of nucleic acid or protein as a sample, and measure the wavelengths 260 nm and 280 nm. The 260/280nm ratio and sample concentration are converted from the measured values. | Nano Volume Plate | |
| Spectrum | Use 2 – 5 µL of nucleic acid or protein as a sample, and measure the spectrum in a wavelength of 240 – 320 nm (2 nm interval). | Nano Volume Plate | ||
| *To use Micro-volume measurement (NANO), ‘Nano Volume Plate’ is required as an option. | ||||
WSL-2300 Phelios AL Offers Simple and Easy ABS Operation
WSL-2300 Phelios AL is a multimode microplate reader for measuring absorbance (ABS), luminescence (LUMI), and microvolume samples (NANO: 2µL). Phelios AL enables the use of a wide range of applications and is also superior in terms of performance and economy.
Key Industries
- Biopharmaceutical and Biotechnology
- Education & Academics
- Medical
- Pharmaceutical
Brand
ATTO Corporation is a scientific instrument developer and manufacturer of protein/nucleic acid research. Long been providing more than 60 years for Polyacrylamide Gel Electrophoresis Devices, PAGEL (precast gel), Western blot solutions, Peristaltic Pumps, Printgraph (High-end Gel Documentation System), Luminograph series (Imaging System for bio/chemiluminescence detection), etc. Their products have been used in many laboratories in Japan and other countries.
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