ATTO – Bioluminescence Measurement System for Living Cells and Tissues – Kronos HT & Kronos Dio
A multi-well plate compatible model, which measures changes in gene expression during cell culture using bioluminescence of luciferase.
Kruno Series has two product models:
- WSL-1565 Kronos HT – WSL-1565 Kronos HT is a high throughput support model of the assay system that measures gene expression of living cells in real-time by using luciferase bioluminescence.
- AB-2550 Kronos Dio – Besides the previous model which was compatible with 35 mm dish measurements, the new Kronos Dio model features multi-well plate measurement functionality with cell culturing capability.
Key Features
- High throughput live cell realtime reporter assay
- Measuring with temperature and CO2 controlled incubation chamber
- 24 well plate x 2 or 96 well plate x 2 (option)
- Data display with real-time
- Detectors scan each well, so culture plates don’t move.
- Lower noise by PMT cooling
- Dual-color luciferase assay by optical filter auto-changing
- Cells and tissue luminescence in 35 mm culture dishes monitoring in real-time
- Adjustable temperatures, CO2 gas levels, and humidity levels
- PMT with incredibly high sensitivity to detect dim and weak bioluminescence
- Up to 3 color bioluminescence measurement at the same time
- Complete control by PC using dedicated software
- Compact design that saves working space
Available Models
Technical Specifications
| Detector | Photomultiplier tube (10°C cooling) x 2 units, X-Y actuating | |
| Measurement | Photon counting method | |
|---|---|---|
| Measurement time | Integral time: 1 – 60 s/well, Measurement term: 1 h – 30 days | |
| Plate format | Clear bottom 24 well plate x 2 Clear bottom 96 well plate x 2 (Option) 35mm dish x 12 (Option) | |
| Throughput | 48 samples (24 well plate x 2) 192 samples (96 well plate x 2) with an optional item, 96well plate adaptor 12 samples (35mm dish x 12) with an optional item, 35mm dish adaptor | |
| Dimensions, Weight | Main unit: 650 (W) x 520 (D) x 340 (H) mm, 40 kg CO2 gas mixer: 160(W) x 300 (D) x 200 (H) mm, 4.8 kg Humidifying unit: 170 (W) x 140 (D) x 115 (H) mm, 1.6kg | |
| View the entire technical specifications in the brochure. | ||
Bioluminescence measurement system for living cell and tissues
The Kronos HT can detect dim and weak luminescence consecutively in 35mm culture dishes for a long time in culture samples such as cells and tissues. The 24×96 well microplates are also compatible.
Our new Kronos HT further strengthens the functions and performance for high throughput measurement by utilizing the know-how cultivated in the precedent model for measuring luminescence while culturing cells. Kronos HT can be used for high-throughput measurements on biological clocks, changes in drug response over time, cytotoxicity tests, and more.
Key Features
- The sample is kept in an incubation chamber with controlled temperature, CO2 concentration, and humidity >90% RH
- 24×96 wells microplate applicable
- Long-time detection is available – 1hour～30days
- 2 plates are measured by 2 PMTs (one PMT to one plate)
- No plates are moved, PMTs are moved to each well – No shaking of cells, so less damage to delicate cells and applicable to floating cells like hemoglobin
- PMTs are cooled to 10℃ – The dark noise is low
- Built-in filters for multi-color luciferase assay
Technical Specifications
| Container | Culture dish, diameter 35 mm | |
| Number of samples | 8 | |
|---|---|---|
| Temperature control | Peltier device and forced air circulation | |
| Temperature range in Kronos Dio incubator | From room temperature – 5ºC to 45ºC in increments of 1ºC (Lowest temp.; 20ºC ) | |
| Detector | PMT (Photomultiplier tube) | |
| Measurement | Photon counting method | |
| Throughput | 8 samples (35 mm culture dish x 8) | |
| Dimensions, Weight | 280 (W) x 400 (D) x 330 (H) mm, 16.0 kg | |
| View the entire technical specifications in the brochure. | ||
Bioluminescence measurement system for living cell and tissues
Kronos Dio (AB-2550) is a luminometer designed to detect bioluminescence in living cells and tissues using a photomultiplier tube (PMT). It features precise temperature and CO2 gas control within the incubator, making it ideal for cell and tissue culturing. The Kronos Dio also offers superior performance for multi-color assays, which can detect up to three colors of luminescence simultaneously with an internal optical filter.
Kronos Dio can detect very dim and weak luminescence continuously for a long time in 35mm culture dishes with its high sensitivity. This eliminates the need to prepare multiple samples for each time point to monitor chronological changes in bioluminescence. As a result, Kronos Dio offers a cost-effective and convenient alternative to conventional methods, saving time while ensuring accuracy and efficiency.
Key Features
- Cells and tissue luminescence in 35 mm culture dishes monitoring in real-time
- Adjustable temperatures, CO2 gas levels, and humidity levels
- PMT with incredibly high sensitivity to detect dim and weak bioluminescence
- Up to 3 color bioluminescence measurement at the same time
- Complete control by PC using dedicated software
- Compact design that saves working space
AB 2550 Kronos Dio Operation Guide
Key Industries
- Biopharmaceutical and Biotechnology
- Education & Academics
- Medical
- Pharmaceutical
ATTO Corporation is a scientific instrument developer and manufacturer of protein/nucleic acid research. Long been providing more than 60 years…
