Trypsin is used to enzymatically release adherent cells from tissue culture plates for passaging.  Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action.  EDTA sequesters these ions and thus enhances the efficacy of trypsin.

A prodigy that’s taking flow cytometry to the next level of performance and flexibility

The Cytek Aurora leverages full spectrum technology to provide unprecedented flexibility to scientists, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomarkers, regardless of assay complexity.

With 500 employees worldwide, Nabertherm has been developing and manufacturing industrial kilns and furnaces for a wide range of applications for over 70 years. As a manufacturer, Nabertherm probably has the widest and deepest range of ovens in the world. 150,000 customers in over 100 countries around the world prove the company’s success. Nabertherm stands for highest quality “Made in Germany”, modern design based on State-of-the-Art technology and a convincing price/performance ratio.

The N1 Single-Cell Dispenser is an accessory to the WOLF^® G2 Cell Sorter

The system uses aerosol-free, sterile, and disposable microfluidic cartridges. It allows for bulk cell selection and sorting directly into 96-well or 384-well plates. The N1 Single-Cell Dispenser seamlessly integrates into the intuitive software, enabling easy selection of single cells from a dropdown menu, plate mapping, and sorting initiation. The WOLF G2 and N1 dispenser collaborate to efficiently sort and plate cells in a compact and cost-effective setup. Post-sorting, cell data are indexed well-by-well for accurate cell confirmation.

The NanoCellect Wolf G2 cell sorter is a powerful tool used for a variety of applications, including antibody discovery, single cell sorting and plating, genomics, single cell RNA sequencing, plant biology, gene editing, cell line development, and discovering microorganisms in ocean or pond water. The Wolf G2 is designed to gently sort happy, healthy cells using a sterile cartridge-based microfluidics system. The Wolf G2 cell sorter is ideal for tasks like cell line development and sorting plant protoplasts, as well as for monoclonal outgrowth of iPSCs and microalgae.