Quantabio

Quantabio reverse transcription reagents combine advanced qScript reverse transcriptase technology in a variety of stabilized, user-friendly formulations that deliver highly reproducible and sensitive RNA detection across a broad range of RNA input.

Our scientists were the first to successfully develop a comprehensive 1-tube reverse transcriptase reagent with sensitivity and linear dynamic range equivalent to traditional component reaction assembly. Quantabio’s new qScript XLT reagents continue to push the envelope of limit of detection (LOD) sensitivity and reproducibility.

Available in a variety of optimized formulations designed to support specific PCR applications and starting materials.

Quantabio PCR reagents are formulated with ultrapure AccuStart DNA polymerase, which contains a stringent antibody hotstart to ensure specific and efficient primer extension only after activation at 94°C.  Our enzymes are rigorously purified to remove host E. coli genomic DNA and are ideally suited for applied testing applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy targets.

Non-specific extension of primers at low temperature is a common cause of PCR artifacts and poor assay specificity and sensitivity.

Q uses a patented magnetic induction technology to rapidly heat samples coupled with fan forced air for cooling to acquire data in only 25 minutes. Available as a 4 channel model, the robust optical system acquires all channels simultaneously and allows for running the fastest multiplexed assays.

Q is exceptionally portable and versatile, without the need for calibration. It scales efficiently, handling up to 48 samples per run, and multiple units can connect wirelessly to a single computer via Bluetooth for processing up to 480 samples simultaneously.

Q’s distinctive spinning aluminum rotor ensures precise temperature control (± 0.05°C), outperforming traditional block-based thermal cyclers with multiple peltier heating blocks that may cause sample variations. This innovation enhances reproducibility and repeatability and enables the detection of 2-fold gene expression differences and precise identification of class IV SNP’s with 0.1°C melt temperature resolution.

PerfeCTa qPCR Reagents combine a stringent, ultrapure antibody hotstart with performance engineered DNA polymerase in stabilized 1-tube formulations optimized for the specific performance needs of real-time quantitative PCR.  Proprietary additives help eliminate persistent bubbles to enable efficient vortex mixing and fewer technical replicates thereby conserving precious sample. Adaptive buffer chemistry accommodates most assay designs and can be used with existing assay designs. PerfeCTa qPCR ToughMix reagents contain proprietary inhibitor-neutralizing additives that relieve several types of PCR inhibition ensuring reliable qPCR assay performance with clinical, plant or environmental specimens or complex food matix.

Quantabio extraction reagents provide a simplified and cost-effective alternative to traditional nucleic acid (NA) purification methods and are optimized to work in series with Quantabio ToughMix reagents.