ABclonal – Molecular Biology Reagents
Our catalog antibodies can be used in popular research areas including epigenetics, DNA damage/repair, cancer, and immunology. Whichever research area you are interested in, our products will provide outstanding results for multiple applications, such as ChIP, ChIP-seq, WB, and IHC. Search our catalog to find the antibodies you need.
Available Models
Technical Specifications
| Components | Component number | Size-1 | Size-2 |
|---|---|---|---|
| 4X ABScript Neo RT Master Mix | RM21486 | 100 μL | 500 μL |
| 20X gDNA Remover Mix | RM21479 | 20 μL | 100 μL |
| Nuclease-free H2O | RM20214 | 1.25 mL | 2 × 1.25 mL |
ABScript Neo RT Master Mix for qPCR with gDNA Remover is an efficient and fast cDNA first-strand synthesis master mix, suitable for two-step RT-qPCR detection. The 4X ABScript Neo RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H2O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.
Technical Specifications
| Components | catalog | Size1 | Size 2 |
|---|---|---|---|
| BrightCycle Universal SYBR Green qPCR Mix with UDG | RM21217 | 5 × 1 mL | 25 × 1 mL |
This product is a universal SYBR Green reagent that uses a specially designed reference dye (ROX), has a more sensitive resolution, and can be applied to all types of fluorescence quantitative PCR instruments on the market (including High ROX, Low ROX, and No ROX required instruments). This reagent introduces a dUTP/UDG anti fouling system. UDG can quickly degrade pollutants containing U at room temperature, and UDG quickly deactivates when pre denatured at 95 ℃, without affecting the efficiency and sensitivity of qPCR. This product uses hot start Taq DNA polymerase for amplification, which greatly improves the specificity of the product while ensuring the amplification effect.
Technical Specifications
| Components | 50 RXN (20 μL/RXN) | 250 RXN (20 μL/RXN) |
|---|---|---|
| Entrans 2X qPCR Probe Master Mix with UDG | 500 μL | 1.25 mL X 2 |
| 50X ROX Dye I | 22 μL | 100 μL |
| 50X ROX Dye II | 22 μL | 100 μL |
Entrans 2X qPCR Probe Master Mix with UDG is a ready-to-use reagent for probe-based qPCR reactions, containing all components except primers, probes and templates. This master mix includes a chemically modified version of Taq polymerase with Hot-start capability to inhibit nonspecific amplification, while allowing high efficiency, sensitivity and reproducibility in qPCR amplification. At the same time, and has joined the UDG antipollution system. The optimization of the Buffer system allows the product to perform multiple fluorescence quantitative experiments, and It is suitable for multiple species and provides a powerful tool for multi-disciplinary experimental needs.
Technical Specifications
| Band | Color | TRIS-GLYCINE / kDa | BIS-TRIS (MOPS) / kDa | BIS-TRIS (MES)/ kDa |
|---|---|---|---|---|
| 1 | Blue | 180 | 170 | 170 |
| 2 | Blue | 140 | 130 | 130 |
| 3 | Blue | 100 | 93 | 93 |
| 4 | Red | 75 | 70 | 72 |
| 5 | Blue | 60 | 53 | 53 |
| 6 | Blue | 45 | 41 | 42 |
| 7 | Blue | 35 | 30 | 30 |
| 8 | Green | 25 | 22 | 23 |
| 9 | Blue | 15 | 14 | 14 |
| 10 | Blue | 10 | 9 | 10 |
The ColorMixed Protein Marker 180 is a ready to use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer).
This marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF nylon, or nitrocellulose) and for approximating the size of proteins.
Notice:
- Readyto use: Do NOT heat, dilute, or add reducing agents before Loading
- Reference bands: 75 kDa (red), 25 kDa (green)
- Loading Volume: 3-5 μL/well for mini gel; 1.5-2.5 μL/ well for Western transfer; 6-10 μL/ well for large gel
- Type of membranes: PVDF nylon, or nitrocellulose
Based in Massachusetts, ABclonal aims to improve the accuracy and precision of life science research for scientists around the world…
